Manual Fluorescence Fluctuation Spectroscopy (FFS), Part A: 518 (Methods in Enzymology)

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I am also grateful to Rudolf Rigler for the workthat he and his colleagues have done that led to the establishment of FCS as a widely accessiblemeasurement method as well as for the many interesting and wide ranging applications thatthey have provided. Lead discovery by miniaturized HTS.

Drug Discovery Today, 3, Mobilitymeasurement by analysis of fluorescence photobleaching recovery kinetics. Fluorescence cross-correlation spectroscopy inliving cells. Nature Methods, 3, Practical guidelines for dual-color fluorescence cross-correlation spectroscopy. Nature Protocols, 2, Single-nucleotide-polymorphism genotyping for whole-genome-amplified samples usingautomated fluorescence correlation spectroscopy.

Analytical Biochemistry, , Two-photon fluorescence correlation spec-troscopy: Method and application to the intracellular environment.

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Dynamic light scattering with applications to chemistry, biology,and physics. Data reduction and error analysis for the physicalsciences. Kinetics of conformational fluctuationsin DNA hairpin-loops. Thermodynamic basis of theenhanced specificity of structured DNA probes. The kinetics of conformational fluc-tuations in an unfolded protein measured by fluorescence methods. Measurement of micro-second dynamic motion in the intestinal fatty acid binding protein by using fluorescencecorrelation spectroscopy.

Probing protein oligomerization in living cellsDigman, M. Mapping the number of37Introduction to FCSmolecules and brightness in the laser scanning microscope. The theory of polymer dynamics. High spatial resolution observa-tion of single-molecule dynamics in living cell membranes. Measurement of membrane proteinlateral diffusion in single cells. Direct observation of the nanoscale dynamics of membrane lipids in a livingcell. Rotational Brownian-motion and fluorescence inten-sity fluctuations.

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  • Fluorescence Fluctuation Spectroscopy (FFS), Part A: Volume 518;
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Chemical Physics, 4, Fluorescence correlation spectroscopy applied to rota-tional diffusion of macromolecules. Quarterly Reviews of Biophysics, 9, Functional roles for noise in genetic circuits. Fluorescence correlation spectroscopy and photobleaching recovery. Annual Review of Physical Chemistry, 36, Quick tour of fluorescence correlation spectroscopy from its inception.

Journal of Biomedical Optics, 9, Biophysical Journal, , A new bio-physical probe based on occupation number fluctuations. Annual Review of Biophysics andBioengineering, 4, Physical Review Letters, 85, Biophysical Journal, 88, One stepfurther in following higher order molecular complex formation. Simultaneous two-photon excitationof distinct labels for dual-color fluorescence crosscorrelation analysis. Breaking the diffraction resolution limit by stimulatedemission: Near-field optical study of protein transport kinetics at a single nuclear pore.

Focal volume optics and experimental artifacts inconfocal fluorescence correlation spectroscopy. Biophysical Journal, 83, Lateral diffusion of proteins in membranes. ElsonAnnual Review of Physiology, 49, Measurement of the translational mobility of con-canavalin A in glycerol-saline solutions and on the cell surface by fluorescence recoveryafter photobleaching.

Biochimica et Biophysica Acta, , Journal of Fluorescence, 17, Statistical accuracy in fluorescence fluctua-tions experiments. European Biophysics Journal with Biophysics Letters, 25, Two-dimensionalfluorescence intensity distribution analysis: Fluorescence-intensity distribution anal-ysis and its application in biomolecular detection technology. Two-photoncross-correlation analysis of intracellular reactions with variable stoichiometry. Advances in image correlation spectroscopy: Measuring number densities, aggregation states, and dynamics of fluorescently labeledmacromolecules in cells.

Cell Biochemistry and Biophysics, 49, Statistical accuracy in fluorescence correlation spectroscopy. PhysicalReview A, 10, Dynamicsof fluorescence marker concentration as a probe of mobility. The techniqueand its applications. Reports on Progress in Physics, 65, Zero-mode waveguides for single-molecule analysis at high concentrations. From subwavelength illumination to nanometric shadowing. Nature Biotechnology, 21, Ligandreceptor kinetics measuredby total internal reflection with fluorescence correlation spectroscopy. Following protein dynamics in living cells.

Dynamics of large semiflexiblechains probed by fluorescence correlation spectroscopy. Physical Review Letters, 90, Uniform translation and laminar flow. Revival of high-order fluorescence correlation anal-ysis: Generalized theory and biochemical applications.

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The Journal of Physical Chemistry. Resolution of fluorescencecorrelation measurements. Biophysical Journal, 76, Single-molecule fluorescence studies of proteinfolding and conformational dynamics. Chemistry Review, , A natively unfolded yeast prion monomer adopts an ensemble of collapsed and rapidlyfluctuating structures. Resolving heterogeneity on the singlemolecular level with the photon-counting histogram. Biophysical Journal, 78, Distribution of resting andligand-bound ErbB1 and ErbB2 receptor tyrosine kinases in living cells using numberand brightness analysis.

Lateral mobility of membrane-binding proteins in living cells measured by total internal reflection fluorescence corre-lation spectroscopy. Biophysical Journal, 91, Reciprocal relations in irreversible processes. Review fluorescence correlation spectroscopy for probingthe kinetics and mechanisms of DNA hairpin formation. Molecular aggregation characterized by highorder autocorrelation in fluorescence correlation spectroscopy. Theory of sample translation in fluores-cence correlation spectroscopy. Biophysical Journal, 51, High-order fluorescence fluctuation analysisof model protein clusters.

Fluorescence intensityand lifetime distribution analysis: Toward higher accuracy in fluorescence fluctuationspectroscopy. Fluorescence intensity multipledistributions analysis: Concurrent determination of diffusion times and molecular bright-ness. Biophysical Journal, 79, A microfluorimetric study of trans-lational diffusion in erythrocyte membranes.

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Scanning fluorescence correlation spectroscopy. Theory andsimulation of aggregation measurements. Biophysical Journal, 49, Quantitation of membrane receptor distributions by image correlation spectroscopy: Biophysical Journal, 65, Confidence intervals for40 Elliot L.

Elsonconcentration and brightness from fluorescence fluctuation measurements. On the statistics of fluorescence correlation spectroscopy. Distribution of molecular aggregation by analysis offluctuation moments. On the analysis of high order moments of fluorescencefluctuations. Biophysical Journal, 57, Analysis of confocal laser-microscope optics for 3-D fluo-rescence correlation spectroscopy. Applied Optics, 30, Fluorescence correlation spectroscopy with high-order anddual-color correlation to probe nonequilibrium steady states.

Chemical fluxes in cellular steady states measured by fluo-rescence correlation spectroscopy. Concentration fluctuations in a mesoscopicoscillating chemical reaction system. Nature, nurture, or chance: Stochastic gene expres-sion and its consequences. Single-molecule approaches to stochastic gene ex-pression. Annual Review of Biophysics, 38, FCS and single molecule spectroscopy. Fluorescence correlation spectros-copy with high count rate and low background: Analysis of translational diffusion.

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European Biophysics Journal, 22, A theory of the linear viscoelastic properties of dilute solutions of coilingpolymers. The Journal of Chemical Physics, 21, Attoliter detection volumes by confocal total-internal-reflection fluorescencemicroscopy. Optics Letters, 29, Confocal microscopy and quantitative single molecule techniques formetrology in molecular medicine.

Mathematik und Naturwissenschaften, Vol. Technischen Universitat Berlin, Berlin. Interstitialcollagenase is a Brownian ratchet driven by proteolysis of collagen. Statistical analysis of fluorescence correlation spectros-copy: The standard deviation and bias. Biophysical Journal, 84, Oligomerization of the EGF receptorinvestigated by live cell fluorescence intensity distribution analysis. Molecular dynamics in livingcells observed by fluorescence correlation spectroscopy with one- and two-photonexcitation.

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Fluorescence fluctuation spectroscopy (FFS). Part A (Book, ) []

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